Latest Research In Enzymes

Research 1

ROLE OF SOME ENZYMES IN THE DEGRADATION OF ACYLUREA COMPOUND, DIFLUBENZURON IN LIPAPHIS ERYSIMI (KALT.)

The mustard aphid,  Lipaphis erysimi (Kalt.), is a serious pest of Brassica crops in India. The leaf surface treatment of chitin synthesis inhibitor, Diflubenzuron (400 ppm) given to its 2nd instar nymphs (48-52h old) for three time intervals 13, 25, 37h,  induced alterations in its enzymatic activity profile. The assessment of the activity of five enzyme systems involved in growth and metabolism revealed that the activity of group transfer enzyme (Glutathione S-transferases), hydrolytic enzyme (Esterases), and oxido-reductase (NADH oxidase) was induced significantly, whereas,  the activity of two other oxido-reductase enzymes (NADH dehydrogenase and Glutathione reductase) showed no specific alterations. It was concluded, that Glutathione S-transferases, NADH oxidase and Esterases might be involved in the degradation of Diflubenzuron in  L. erysimi.

Research 2

SECRETION OF PECTIC ENZYMES BY ALTERNARIA SOLANI AND ALTERNARIA TRITICINA

The present study deals with the effect of monosaccharide (Glucose) and polysaccharide (Starch) on the production of pectic enzymes (endo-PGTE, endo-PMTE, endo-PG. endo-PMG and PME) in the inoculum of Altarnaria solani on tomato and Altarnaria triticina on wheat in modified Richard's culture medium. Secretion of pectic enzymes as stimulated with monosaccharide by both pathogens but in case of polysaccharide the secretion of pectic enzymes was repressed by both pathogens under similar conditions. The stimulation and repression of pectic enzymes by Alternaria solani and Alternaria triticina depend not only on the nature of pathogen and the host but on the structure of carbo-hydrates.

Research 3

DETERMINATION OF PREVALENCE OF MALIGNANT CATARRHAL FEVER BY USING COMPETITIVE INHIBITION ELISA IN DOMESTIC CATTLE

The aim of this study was to determine the seroprevalence rate of Malignant Catarrhal Fever (MCF) in domestic cattle in Konya. In this study, serum samples were collected from 189 domestic cattle from private five farms in Konya and its environment. Competitive Enzyme Linked Immunosorbent Assay (CI-ELISA) was used for detection of antibodies against MCF virus. 23 (12.16%) of total 189 blood samples were found to be seropositive and 166 (87.83%) as seronegative against MCF infection using by CI-ELISA. This is the first time that seroprevalence of MCF have been studied in domestic cattle in Konya.