Leaf and internodal explants of Physalis angulata L. were used to evaluate the
effect of growth regulators on the in vitro callus induction and plant
regeneration. Two independent experiments were performed to establish callus
and plant regeneration. In the first experiment leaf segments 0.5mm were
cultured on MS basal medium fortified with (BAP (0.5-4.0 mg/l) + 2, 4-D
(0.0-1.5 mg/l). Callus initiation was observed best in all media after 12-16
days. Highest callus growth in terms of 11.75% Yellowish Green Friable Callus
(YGFC) was observed in MS medium fortified with 2, 4-D 1.0 mg/l and BAP 2.0
mg/l. In the second experiment internodal segments 0.5 mm were cultured on MS
medium containing BAP (0.5-4.0 mg/l) + IAA (0.5-3.0 mg/l) + KIN (1.0 -3.0 mg/l)
and 2, 4-D (0.5-3.0 mg/l). The best response of shoot induction was observed
with internodal cultured onto MS medium containing BAP (2.0 mg/l) + KIN (1.0
mg/l) and IAA (1.0 mg/l) showed the mean value 14 Number of shoots per explant.
Excised shoots were transferred to rooting medium containing different
concentrations of IBA, IAA and NAA for root induction. The roots were initiated
and well developed on a medium fortified with 0.5-5.0 mg/l of both the auxins.
Of the three auxins tested, a number of roots were produced on the medium
containing IBA. Maximum numbers of 35 roots per shoot were produced in 35 days
on MS medium supplemented with 3.0 mg/l IBA. The well-rooted plantlets were
transplanted to the paper cup for hardening and the well-established plants
were transferred to the field for acclimatisation.
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