The study investigated the antibacterial effect of honey
against uropathogenic bacteria isolated from clinical samples (Escherichia
coli, Morganella morgani, Enterobacter cloacae, Klebsiella pneumoniae, Serratia
fonticola, Proteus mirabilis and Pseudomonas aeruginosa). A total of four
natural Algerian honeys (NAHs) were investigated for their antibacterial
activity against some uropathogenic isolates. They were analyzed for their
color, total phenolic content (TPC) and total flavonoid content (TFC). Two
different assays were performed to evaluate the antibacterial potential of the
NAH samples: agar-well diffusion and disc diffusion methods. Undiluted and
two-fold serial dilutions of NAH (50, 25 and 12.5%) were tested to determine
zone of inhibition diameters (ZID). Antibiotic susceptibility profiling were
performed according to CA-SFM (Antibiogram committee of French society of
microbiology). Results showed that TPC values ranged from 0.682 mg GAE/g for
NAH1to 0.510 mg GAE/g for NAH2. TFC ranged from 0.166 mg QE/g (NAH1) to 0.102
mg QE/g (NAH4).The color intensity of the honey samples ranged from 1.10±0.11
to 1.44±0.03 mAU. The diameter of ZDI ranged from 7.5 to 13 mm for Escherichia
coli, 8–13 mm for Morganella morgani, 6.8-13.40 mm for Klebsiella pneumoniae,
8-13.4 mm for Enterobacter cloacae, 8-13.5 mm for Serratia fonticola, 7.4-14.5
mm for Proteus mirabilis and 7.6-13.2 for Pseudomonas aeruginosa. The highest
activity was induced by NAH3, followed by NAH1, NAH4, and NAH2. The clinical
isolates of enterobacteriaceae had a higher resistance profile than other
Gram-negative bacteria for most commonly prescribed antibiotics. The overall
results of this study indicated that Algerian honey could be used to treat
urinary tract infections (UTIs) caused by the tested bacteria.
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