Background:
Tabebuia genera had been reported several pharmacological activities. Cuba has
numerous endemic species in this genera, but only our group reported the
anti-inflammatory, in vitro and in vivo antiproliferative properties from the
specie Tabebuia hypoleuca (C. Wright) Urb. In vitro studies demonstrated that
786-0 (kidney), MCF-7 (breast) and glioma (U-251) were among the cancer cell
lines most sensitive to ethyl acetate extract of leaves treatment.
Purpose: The
aim of this study was to research the induction of cell cycle arrest and
apoptosis by a pentacyclic triterpenes -rich fraction from Tabebuia hypoleuca
leaves in U- 251 glioblastoma cells and identify the triterpene structures in
this fraction.
Methods: A
rich fraction of pentacyclic triterpenes were proceeded for the cell cycle
arrest and apoptosis on glioblastoma cells using Annexin V and flow cytometry.
The chemical composition was determinate by chromatography and spectroscopic
methods.
Results: Our results showed that a pentacyclic
triterpenes –rich fraction had the ability to cause cell arrest in G2/M phase
and induce apoptosis. The elucidation of the chemical compounds in this
fraction revealed a mixing of oleanolic, ursolic and betulinic acids. This
finding about chemical composition in the first report by the genera.
Conclusion:
Therefore, this study showed the presence of different pentacyclic triterpenes
in T. hypoleuca and it have promising anticancer activity against glioblastoma.
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