The Changes in protein content and peroxidase activity were observed in two species of Kalanchoe, namely K. pinnata and K. daigremonitiana, while anatomical changes were observed in K. pinnata only. The drifts were observed applying chloramphenicol, a putative inhibitor of protein synthesis. In K. pinnata, total protein content rose to high level twice with initiation of rooting and sprouting. In K. daigremontiana, the protein content showed regular rhythmic fluctuations except for an upsurge just prior to rooting. The total peroxidase activity during differentiation indicates that this enzyme system is not directly involved in a significant way. Histological changes revealed different stages of cellular arrangements during differentiation.
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