SEROLOGICAL AND MOLECULAR DIAGNOSIS OF Toxoplasma gondii | BIONATURE

This study aimed to determine the immune status of pregnant women at the Central Hospital of Ain Naâdja (HCA) in Algeria. For this purpose, the identification of anti-Toxoplasma immunoglobulins IgG and IgM by ELFA test was carried out in 393 patients, as well as the comparison of the serological profile for the mother-child, serum-aqueous humor pairs by the Western Blot (WB) assay. Parasite search was done by standard PCR on placenta samples using JW62/JW63 primer. The results obtained from 393 serums collected showed that: 40.96% of the pregnant women having a serological profile in favor of an old Toxoplasmosis, and 59.03% were not immunized (P<0.05). Serological comparison by WB analysis of the pairs revealed that 90.1% of the couples possessing the same serological profile (negative), and 9.09% were positive (congenital toxoplasmosis) (P<0.05). To conclude, The PCR performed on placentas showed a high level of specificity for diagnosis. It allows early diagnosis and should be recommended for identification of congenital infection.

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