The antimicrobial activity of propolis extracts is well documented. Nevertheless, the properties of the Algerian propolis are poorly investigated. This fact could be due to the diversity of the climate and the geographic areas from which propolis is usually harvested. The present work is a contribution to evaluate the antimicrobial susceptibility testing of propolis varieties with high total polyphenols and flavonoids contents, chosen beforehand from an analysis made of 10 propolis samples. The disk diffusion method was employed to determine the antistaphylococcal activity of three EEAP (ethanolic extract of Algerian propolis) samples, at different concentrations (5%, 10, 20 and 30%), against a reference bacterium strain of Staphylococcus aureus, the most redoubtable agent in osteomyelitis. Inhibitory zone diameters for the tested bacterium were 14.99 mm for EEAP at 5% concentration, 16.41 mm at 10%, 18.08 mm at 20% and 19.16 mm at 30%, respectively. Our in vitro study revealed that EEAP with high polyphenols and flavonoids contents exhibit a strong antibacterial activity, and it may be indicated as an alternative solution for the treatment of staphylococcal osteomyelitis.
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Keywords: Algerian propolis, Staphylococcus aureus, osteomyelitis, inhibition zone
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