Morphological traits and molecular markers analyses are very
important tools for estimation of genetic variability among genotypes. In this
study, we combined the phenotypic characterization with the inter-simple
sequence repeats (ISSR) technique for the assessment of genetic diversity among
26 bread wheat genotypes. Out of 87 amplified bands 43 were polymorphic. The %P
ranged from 25 to 76.92 with an average of 46.97%. The average number of
polymorphic bands was 4.30 per primer. UBC-849 and UBC-833 primers which
belonged to (GT) and (GA) repeats primer groups produced 62.50 and 76.92% level
of polymorphism, respectively. It is clear that the highest polymorphism level was
obtained in the case of di-nucleotide repeat primers. In this work the
polymorphism information content (PIC) values varied from 0.04 (UBC-819) to
0.19 (UBC-834) with an average of 0.13. The Marker index (MI) values were from
0.12 (UBC-819) to 1.50 (UBC-833) with an average of 0.62. Two markers
(UBC-811930bp and UBC-833600bp) were identified which may be linked to stem
diameter and grain yield per plant traits, respectively.
Please read full article –https://globalpresshub.com/index.php/AJORIB/
Keywords: Triticum aestivum L., molecular markers, grain
yield, polymorphism
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