IDENTIFICATION, ISOLATION AND in silico CHARACTERIZATION OF Fragaria vesca HOMOLOGUE OF TEMPRANILLO factor

The ground strawberry, Fragaria vesca belongs to the Rosaceae family, one in all the foremost economically vital family of flowering tree crops, commonly with long juvenility amount. Therefore, information regarding the molecular basis of flowering time regulation and photoperiodic responses in these species is important for breeding goals. Shortening the juvenility amount has been delineated  as a motivating breeding goal for many of the fruit trees, that may well be achieved through recombinant DNA technology. TEMPRANILLO (TEM) belongs to the RAV1 (Related to ABI3/VP1) transcription factors family, that is thought as a flowering agent, containing AP2 and a B3 DNA-binding domains. during this study, we have a tendency to determine and isolate F. vesca homologue of Arabidopsis TEM (AtTEM). The full-length complementary DNA of FvTEM consisting of a writing sequence of 1152 bp, foretold to cipher 383 amino acids was obtained, cloned and more analyzed in-silico. victorious biological research was confirmed exploitation colony PCR and sequencing. Bioinformatic analysis showed that the promoter region of FvFT1 contains CAACA and CACCTG motifs that may well be recognised as binding sites for FvTEM and directly represses FvFT1 expression. In-silico expression analysis in F. vesca Eastern Time information found twenty one ESTs with identities over fiftieth. phyletic analysis exploitation neighbor connection methodology unconcealed that FvTEM is homologous with AtTEM.

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