In order to better
understand the relationship genotype-phenotype, marker-trait associations were
studied in eight wheat genotypes using a set of 95 DNA-based SRAP molecular
markers. SRAP analysis showed that the average of the percentage of
polymorphism (P%) was 62.59%, as well as the average of polymorphic information
content (PIC) was 0.23. Moreover, the means of marker index (MI) was 1.49. The
ME1F-EM5R and ME9F-EM3R primers combination showed higher levels of
polymorphism of 88.89% and 90.91%, respectively. Single-marker analysis (SMA)
indicated that ME-7F-EM-6R1250bp was probably as a candidate marker which
linked to 1000-grain weight. In addition, ME-7F-EM-5R900bp and ME-4F-EM-6R490bp
SRAP markers were identified for weight of grains/spike and No. of
grains/spike, respectively. The cluster analysis based on SRAP and means of
morphological data revealed similarity coefficient values ranged from 57.40% to
80.40% and from 90.61% to 98.81%, respectively. Similarity matrices generated
by SRAP markers and means of morphological traits showed a positive but
non-significant correlation (r = 0.03, P˃ 0.05).
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