Callus proliferation was studied on leaf of explant of
Spondias mangifera L. cultured on Murashige and Skoog's medium (MS)
supplemented with auxin (2, 4D, IBA, IAA and NAA) and cytokinin (BAP) alone or
in combination. Most suitable medium noted for shoot formation from leaves was BAP (2.5 ppm) in MS medium. Shoot
developed was rooted the best in MS medium with 0.5 ppm NAA.
Culture kept under various conditions of light and temperature to find
the suitable physical condition for the best growth.
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