Use of micropropagation in the production of
bamboo plants plays important role. Successful acclimatization is the key
factor for efficient and large scale production. For efficient hardening,
proper understanding of leaf surface structure is necessary because microshoots
developed in vitro have different morphology than in vivo, Scanning electron
microscopy was performed to compare the ultrastructural variation in leaves of
bamboo (Dendrocalamus asper) grown in vitro and in vivo. Leaf samples were taken at four stages - in
vitro, unhardened, acclimatized and mother plant. Ultrastructural studies
revealed substantial differences in the morphology of in vitro grown plant and
mother plant but unhardened and acclimatized plants did not show much variation
from in vitro condition. Variation in the presence of stomata, trichomes, papillae
and prickle hairs was observed on abaxial and adaxial surface.
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